MOBILITY STUDY OF DNA COVALENTLY LINKED TO DIFFERENT FLUOROPHORES IN PDMS MICROCHANNELS.

B. LERNER; M.S. PEREZ; A.F. ORDOÑEZ ARIAS; F. SACCO; M.J. DIEGUEZ; B. CETRALONGO; C. LASORSA; C.A. RINALDI; A. LAMAGNA

Nuremberg

Congreso; IMCS 2012,14th International Meeting on Chemical Sensors; 2012

In this work electrophoresis experiments were performed on PDMS microchannels, also the work analyzed the mobility and separation of DNA molecules in Polydimethylsiloxane (PDMS) microchannels filled with agarose. Fluorescence microscopy was implemented for the detection of DNA molecules covalently attached to fluorophores. The mobility and diffusion of DNA molecules was characterized at voltages up to 1.8 kV. The work also shows the application in the separation of DNA molecules with lengths of between 0.5 and 2kpb.