INVESTIGADORES
LERNER Betiana
congresos y reuniones científicas
Título:
FUNCTIONALIZATION OF CARBON NANOTUBES WITH BIOMOLECULES FOR NANOBIOSENSORS TO DETECT CANCER MARKERS
Autor/es:
TROPPER IVANA; VILLAGRASA LUCILA; LERNER BETIANA; PÉREZ MAXIMILIANO; BOSELLI ALFREDO; LAMAGNA ALBERTO; POLICASTRO LUCILA; HEBE DURAN
Lugar:
Puerto Madryn, Chubut
Reunión:
Congreso; Argentine Society for Biochemistry and Molecular Biology; 2010
Resumen:
The aim of this study was to functionalize carbon nanotubes
(CNTs) with antibodies or oligonucleotides to produce
nanobiosensors for tumor markers detection. For this purpose, first
CNTs were oxidized by incubation with a mixture of acids. Then
oxidized CNTs (CNTs-COOH) were covalently bound to
antibodies or modified oligonucleotides with an amine group at 5
end by incubation with 1-ethyl-3-(dimethylaminopropyl)
carbodiimide (EDC) and N-hidroxysuccinimide (NHS). The
product of this reaction is an amide bond between a carboxyl group
of CNTs-COOH and an amine group present in the proteins or in the
modified oligonucleotides. The products of both steps of this
functionalization were characterized by scanning electron
microscopy (SEM), Raman spectroscopy and thermogravimetric
analysis (TGA). SEM images and Raman spectra showed the
characteristic differences on morphology and Raman peaks
between CNTs and CNTs-COOH. TGA analysis demonstrated the
presence of the organic molecules (protein or DNA) and the degree
of functionalization in the samples of CNTs-protein and CNTs-
DNA. Thus, these techniques allowed the characterization of CNTs
functionalization with biomolecules to be used in the development
of electronic nanobiosensors devices for specific cancer markers
detection.