DNA ELECTROPHORESIS THROUGH MICROPORES MANUFACTURED BY LASER ABLATION

M.S. PEREZ; B. LERNER; P.A. KLER; C.L.A. BERLI; J.A. LELL; A. FASCISZ ZEBALLOS; B. CETRALONGO; C. LASORSA; C.A. RINALDI; A. LAMAGNA

Nuremberg

Congreso; IMCS 2012,14th International Meeting on Chemical Sensors; 2012

The work presents an alternative method for the manufacture of micropores by using the combination of laser ablation and wet etching. The fabricated pores were then integrated to polydimethylsiloxane (PDMS) microchannels, which constitutes a novel setup to be considered for microfluidic applications. Furthermore, as the system is addressed to study the electrically-driven transport of macromolecules, deoxyribonucleic acid (DNA) electrophoresis was performed in the fabricated microsystems to prove the principle. Also the electric field throughout the microchannel and pore region was studied in details by using numerical simulations.