Modulation of sphere-forming stem-like cell populations with chemotherapeutic drugs using a murine mixed cellular breast cancer model

AGÜERO, EDUARDO IMANOL; BELGOROSKY, DENISE; LERNER, BETIANA; PÉREZ, MAXIMILIANO SEBASTIÁN; EIJÁN, ANA MARÍA

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Congreso; Reunión anual de sociedades de biociencias SAIC. SAI. SAFIS; 2020

Introduction: Breast cancer (BC) is the tumor with the highest incidence in women and a significant cause of cancer-related morbidity and mortality worldwide. The standard treatment depends on several factors including stage, histology and expression of molecular markers. Adjuvant or neoadjuvant therapy are mechanisms that facilitate tumor resection and chemotherapy (CT) is a widely approach used many times as concomitant neoadjuvant therapy with radio, endocrine or immunotherapy. Cancer Stem Cells (CSC) are a minority tumor cell population, associated to the lack of treatment response. The development of an in vitro assay that can predict treatment response and that could be useful for patients with this pathology is an important issue. Objective: Evaluate CSC response post-treatment CT drugs like doxorubicin (Doxo) and paclitaxel (Ptx) using a murine BC mixed cell line (LM38-LP). Results: Through a cell viability assay in monolayer, measured by MTS, IC50 values were obtained, being 1,5 µM for Doxo and 0,5 µM for Ptx. Cancer sphere forming assay in low-attachment conditions, an accepted method for CSC quantification, was carried out with or without Doxo or Ptx. Both CT compounds decreased the number of CSCs, measured as sphere formation efficiency