Diazepam inhibits acute innate and ongoing adaptive inflammatory responses

FALCÓN CRISTIAN; MONFERRÁN CLARA G; CERVI LAURA; ROTH GERMÁN A.

Mar del Plata

Congreso; LXII REUNIÓN ANUAL Sociedad Argentina de Inmunología; 2014

Sociedad argentina de inmunología

In addition to the central GABAergic receptors described forbenzodiazepines, peripheral-type benzodiazepine receptor (PBR)was also identified for these molecules in immune cells, such asmacrophages and lymphocytes. PBR activation was reported todecrease inflammatory immune responses. In this regard, we previouslydemonstrated that Diazepam (Dz) decreases LPS-induceddendritic cell activation and their ability to induce allogeneicresponses, thus preventing the initiation of immune responses.In this work we demonstrate that Dz is able to diminish acuteinnate and adaptive ongoing inflammatory responses in vivo. Forthis, we treated C57BL/6 mice with Dz (2mg/Kg) at days 0, 2 and4, and 12 h after the last treatment the mice were injected (i.p.)with 800 μg de LPS. Dz was able to prevent LPS-induced deathand inhibit the IL-6 secretion (p