Role of programed death ligand 2 in macrophages activation during Fasciola hepatica infection

STEMPIN CINTHIA; FALCÓN CRISTIAN; AOKI PILAR; MOTRAN CLAUDIA CRISTINA; CERBAN FABIO; CERVI LAURA

Mar del Plata

Congreso; LXII REUNIÓN ANUAL Sociedad Argentina de Inmunología; 2014

Sociedad argentina de inmunología

Helminth infections induce an increase in co-inhibitory moleculeprogrammed death ligand-2 (PD-L2) which is defined as a markerfor alternatively activated macrophages and is involved in the inhibitionof T cell proliferation. However, it is not known how PD-L2modulates macrophage activation, which promotes fibrosis in helminthinfections, whereas classical activation produces deleterioustoxic metabolites. Previously we have observed that F. hepaticainfection increases PD-L2 expression in F4/80+ cells. The aim ofthis study was to investigate the role of PD-L2 in the outcome ofF. hepatica infection as well as in macrophage activation duringexperimental infection with the parasite. BALB/c WT and PD-L2KO were orally infected with 8 F. hepatica metacercariae. Survivalrate was evaluated and histological changes in liver tissues weredetermined by hematoxylin and eosin staining. PD-L2 KO miceshowed increased mortality during F. hepatica infection (p