INVESTIGADORES
FALCON cristian roberto
artículos
Título:
Excretory-secretory products (ESP) from Fasciola hepatica induce tolerogenic properties in myeloid dendritic cells
Autor/es:
FALCÓN CRISTIAN; CARRANZA FRANCO; MARTINEZ FERNANDO; KNUBEL CAROLINA; MASIH DIANA; MOTRÁN CRISTINA; CERVI LAURA
Revista:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2010 vol. 137 p. 36 - 46
ISSN:
0165-2427
Resumen:
Fasciola hepatica is a helminth trematode that migrates through the host tissues until reaching
bile ducts where it becomes an adult. During its migration the parasite releases different
excretory-secretory products (ESP), which are in contact with the immune system. In this
study, we focused on the effect of ESP on the maturation and function of murine bone
marrow derived-dendritic cells (DC). We found that the treatment of DC with ESP failed to
induce a classical maturation of these cells, since ESP alone did not activate DC to produce
any cytokines, although they impaired the ability of DC to be activated by TLR ligands and
also their capacity to stimulate an allospecific response. In addition, using an in vitro ovalbumin
peptide-restricted priming assay, ESP-treated DC exhibited a capacity to drive Th2
and regulatory T cell (Treg) polarization of CD4+ cells from DO11.10 transgenic mice. This
was characterized by increased IL-4, IL-5, IL-10 and TGF-_ production and the expansion
of CD4+CD25+Foxp3+ cells. Our results support the hypothesis that ESP from F. hepatica
modulate the maturation and function of DC as part of a generalized immunosuppressive
mechanism that involves a bias towards a Th2 response and Treg development