Modification of Band 3 and oxidation level of membrane protein in senescent erythrocytes

RUCCI A; ENSINCK A; MUFARREGE N; COTORRUELO C; GARCÍA BORRÁS S; RACCA L; BIONDI C; RACCA A

CLINICAL BIOCHEMISTRY

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2010 vol. 43 p. 1171 - 1173

0009-9120

Objectives: To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). Design and methods: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups. Results: Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC. Conclusions: These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan.