Senescent Erythrocytes: Modification Of Rheologic Properties, Antigenic Expression And Interaction With Monocytes.

RACCA A; BIONDI C; COTORRUELO C; GALIZZI S; RASIA R; STOLTZ J; VALVERDE J

MEDICINA (BUENOS AIRES)

Fundación Revista Medicina

Lugar: Buenos Aires- Argentina; Año: 1999 vol. 59 p. 33 - 37

0025-7680

Human erythrocytes have a well-defined lifespan of 120 days. Their eventual removal from circulation is a complex process affected by many cellular parameters, making them susceptible to sequestration in the spleen and other organs. The purpose of this study was to investigate putative changes in rheologic properties, antigenic expression and interaction with monocytes of senescent erythrocytes (SE). SE and young erythrocyte (YE) fractions were obtained by differential centrifugation from 20 healthy donor blood samples. Membrane rheomechanic properties (by difractometric mathod), ABO and MN antigens reactivity and erythrophagocytosis by peripheral monocytes were investigated in each fractions. SE showed a little decrease in the deformability index, and an increase of both membrane elastic modulus and surface viscosity. The studies performed indicated a decreased expression in the antigens of both blood group systems studied (p