Lipoteichoic acid from Lacticaseibacillus rhamnosus GG modulates dendritic cells and T cells in the gut

FRIEDRICH ADRIAN; LEONI, JULIANA; PAZ, MARIELA LAURA; GONZÁLEZ MAGLIO, DANIEL H.

Nutrients

MDPI

Año: 2022

2072-6643

Lipoteichoic acid (LTA) from gram-positive bacteria exerts different immune effects depending on the bacterial source from which it is isolated. Lacticaseibacillus rhamnosus GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mice. In the present work, we evaluate the immunomodulatory effect exerted by LGG-LTA in dendritic cells (DC) and T cells, both in vitro and in the gut-associated lymphoid tissue (GALT).During cell culture, LTA- stimulated BMDC increased CD86 and MHC-II expression and secreted low levels of pro and anti-inflammatory cytokines. Moreover, LTA-treated BMDC increased T cell priming capacity, promoting the secretion of IL-17A. On the other hand, in orally LTA treated mice, a decrease in mature DC (lamina propria and Peyer?s patches) was observed. Concomitantly, an increase in IL-12p35 and IFN-γ transcription was presented (lamina propria and Peyer?s Patches). Finally, an increase in the number of CD103+ DC was observed in Peyer?s patches.Together, our data demonstrate that LGG-LTA activates DC and T cells. Moreover, we show that a Th1 biased immune response is triggered in vivo after oral LTA administration. These effects justify the oral LTA activity previously observed.