Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet- Irradiated Skin in Five Different Mice Strains

FRIEDRICH, ADRIÁN; CELA, ELIANA; PAZ, MARIELA; LEONI, JULIANA; GONZÁLEZ MAGLIO, DANIEL

Global Journal of Dermatology & Venereology

Karachi : Synergy Publishers

Año: 2014

Ultraviolet radiation (UVR) effects on skin have been extensively studied. Several mice strains have beenused worldwide in photobiology and photoimmunology studies. Recently, we have developed a method based on flowcytometry in order to analyze mitochondrial dysfunction and superoxide (O2?-) production ex vivo in keratinocytes isolatedfrom irradiated-mice. This method can be helpful to evaluate mitochondrial alterations in different mice models.In this work, we aimed to compare epidermal response to UVR in five mice strains, both pigmented and albino as well ashairy and hairless strains (SKH:1, Balb/c, C57/BL, DBA/2N and Swiss). Keratinocytes mitochondrial alterations,epidermal hyperplasia and inflammatory mediators? production (epidermis and serum) were determined 72 hours after a400 mJ/cm2 UV dose.All strains showed epidermal hyperplasia and loss of mitochondrial polarization after irradiation, differing in themagnitude of the response. However, there were significant differences in the basal mitochondrial polarization betweenstrains, showing that the metabolic state of keratinocytes may vary between them. Moreover, mitochondrial O2production was induced in SKH:1 and Balb/c after irradiation, whereas in DBA/2N, Swiss and C57/BL it was at the samelevel or even lower than in the non-irradiated control. Finally, an increase in inflammatory mediators was only detected inthe serum of C57/BL and Swiss mice and in the epidermis of DBA/2N and C57/BL.Results show that each mice strain has particular characteristics related to cellular metabolism, which may lead toparticular responses to UVR exposure. Therefore, the use of a particular mice strain in photobiology models should becarefully considered