Modulation of peptidoglycan recognition proteins­mediated porcine intestinal innate immunity by paraimmunobiotic bifidobacteria. LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología

MA ISLAM; H IIDA; M TOHNO; R OHGI; LEONARDO ALBARRACÍN; W IKEDA­OHTSUBO; A MIYAZAKI; H UENISHI; Y SUDA; N IWABUCHI; J XIAO; J VILLENA; H KITAZAWA

San miguel de tucuman

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Peptidoglycan recognition proteins (PGLYRPs) are able to induce innate immune responsesthrough their binding to peptidoglycan (PGN) or by interacting with other pattern recognitionreceptors (PRR)-ligands in the intestinal mucosa. Recently, progress has been made inunderstanding the immunobiology of PGLYRPs in human and mice, however their functions inlivestock has been less explored. In this study, the expression patterns of PGLYRPs in porcineintestinal epithelial (PIE) cells and antigen presenting cells (APCs) were characterized. Inaddition, the modulation of PGLYRPs activation by the interactions of host cells with PRRligandsand non-viable immunomodulatory bifidobacteria (paraimmunobiotic bifidobacteria)was studied. Immunofluorescence and qPCR analysis showed that PGLYRP-1, -2, -3 and -4 areexpressed in PIE cells and APCs from Peyer´s patches, being PGLYPR-3 and -4 levels higher thanPGLYRP-1 and -2 (p