Postimmunobiotics increase antiviral immunity in alveolar macrophages: impact on the resistance against respiratory syncytial virus

F RAYA TONETTI; R ORTIZ MOYANO; DENTICE MAIDANA, STEFANIA; ELEAN, MARIANO; ALBARRACÍN, LEONARDO MIGUEL; H KITAZAWA; VILLENA JULIO

Congreso; Reunión Conjunta SAIC - SAI - FAIC - SAFIS 2022; 2022

Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in the prevention of respiratory viral infections. In this study, we evaluated the ability of 36 Lactobacillus gasseri strains to modulate the immune response of porcine alveolar macrophages (3D4/31 cells) to the stimulation with the Toll-like receptor 3 (TLR3) agonist poly(I:C). By determining the expression of IFN-β and RNAseL we selected L. gasseri TMT36, TMT39 and TMT40 as the strains with immunomodulatory potential. Postimmunobiotics (HK) derived from the heat treatment of those strains were also evaluated in macrophages. In our hands, HK36, HK39 and HK40 enhanced the expression of IFN-β, IFN-λ1, and the antiviral factors RNAseL and Mx2 (p