Effect of immunobiotic bifidobacteria on the innate antiviral immune response in bovine intestinal epithelial cells: immunotranscriptomic analysis

ALBARRACÍN, LEONARDO MIGUEL; AKM HUMAYUN KOBER; HISAKAZU KOBAYASHI; NANA SATO; PAULRAJ KANMANI; WAKAKO IKEDA-OHTSUBO; TOMONORI NOCHI; HISASHI ASO; NORIYUKI IWABUCHI; JIN-ZHONG XIAO; TADAO SAITO; JULIO VILLENA; HARUKI KITAZAWA

Congreso; 7th Congress of European Microbiologists (FEMS 2017); 2017

BackgroundsPrevious studies showed that Bifidobacterium infantis strain or B. breve strain significantly reduced rotavirus titers in infected bovine intestinal epithelial (BIE) cells.ObjectivesIn this work, transcriptomic studies in BIE cells were performed to advance in the understanding the mechanisms involved in the antiviral activity of bifidobacteria.MethodsBIE cells were stimulated with B. infantis or B. breve (5 x 108 cells/ml) and then challenged with the TLR3 agonist poly(I:C). BIE cells with no bifidobacteria treatment were used as controls. The transcriptomic response was evaluated 12 h after poly(I:C) challenge using the Bovine (G3) Gene Expression Microarray (Agilent). Variations in transcript abundance with a t-test p