Corynebacterium pseudodiphteriticum STIMULATE ALVEOLAR MACROPHAGES AND IMPROVE THE RESPIRATORY HUMORAL IMMUNE RESPONSE

ORTIZ MOYANO, RAMIRO; RAYA TONETTI, FERNANDA; ELEAN, MARIANO; DENTICE MAIDANA, STEFANIA; ALBARRACÍN, LEONARDO; ALVAREZ, SUSANA; VILLENA, JULIO

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SAIC SAIyFAIC SAFIS

Previously, we demonstrated that nasally administered Corynebacterium pseudodiphteriticum 090104 (Cp) or its bacterium-like particles (BLPCp) were able to increase the resistance of mice against bacterial and viral respiratory pathogens. In this work, we evaluated: a) the interaction of Cp and BLPCp with alveolar macrophages (AMs) and also b) their ability to enhance the humoral immune response induced by a commercial vaccine against Streptococcus pneumoniae (Sp). First, Cp or BLPCp were incubated with primary cultures of murine AMs, the phagocytic activity and the production of cytokines were evaluated. Optical and electron microscopy analysis revealed that Cp and BLPCp were efficiently phagocyted by AMs. Plus, they were able to trigger the production of TNF-α, IFN-γ, IL-6 and IL-1β (p