POTENTIAL NEUROPROTECTIVE EFFECTS ASSOCIATED TO IMMUNE MODULATION BY SELECTED LACTIC ACID BACTERIA IN MODELS OF PARKINSON'S DISEASE

DAIANA PEREZ VISÑUK; MARÍA DEL MILAGRO TERÁN; GRACIELA SAVOY DE GIORI; JEAN GUY LEBLANC; MARÍA ALEJANDRA DE MORENO

San Miguel de Tucumán

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Parkinson´s Disease (PD) is a neurodegenerative process affecting motor function that involves an inflammatory response and B group vitamin deficiencies. Aim: To evaluate the effect of B group vitamin producing and immunomodulatory lactic acid bacteria (LAB) in murine models of PD. Methods. Lactobacillus plantarum (Lp) CRL2130, Streptococcus thermophilus (St) CRL808, and Lp CRL1905 (riboflavin, folate and thiamin producers, respectively), and St CRL807 (immunomodulatory strain) were evaluated individually or as mixtures using in vitro and in vivo PD models. N2a neuronal cells were exposed to the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) in presence of intracellular extracts from vitamin producing LAB. Cell viability and IL-6 production were determined. In vivo, LAB were administered to mice injected with 1-meti-l-4-fenil-1,2,3,6-tetrahidropiridina (MPTP). Motor capacity, tyrosine hydroxylase (TH) in brain, and serum and brain cytokines were evaluated. Results: Neurotoxic effect of MPP+ decreased in cells cultured with LAB intracellular extracts. This was associated to decreased values of IL-6. Mice given LAB improved motor tests and increased brain TH+ neurons compared to PD control group. LAB effect was associated to decreased IL-6 and TNF-alpha, and increased MCP1 in the serum of the mice. MPTP also increased these cytokines locally in mouse's brains where LAB could also exert benefits. The individual effect of each LAB was improved when the mice received the LAB mix demonstrating the importance of adding different mechanism of action. Conclusions: LAB selected as B group vitamins producers or as immunomodulators have potential to be used as immune adjuvants and perhaps as intestinal microbiome modulators against PD.