BEHAVIOUR OF BENEFICIAL LACTIC ACID BACTERIA (BLAB) IN THERMAL CONDITIONS FOR THEIR TECHNOLOGICAL APPLICATION.

J. A. SILVA; A. MARCHESI; M. E. F. NADER-MACÍAS

Buenos Aires

Encuentro; Reunión Conjunta de Sociedades de Biociencias; 2017

Comité Organizador de la Reunión Conjunta de Sociedades de Biociencias

Lactic Acid Bacteria isolated from vaginal health woman, had demonstrated diverse beneficial properties that improve the urogenital physiological balance , to be included in pharmaceuticals products is important to maintain their viability and to be able to have a prolonged shelf life, it is necessary to subject them to drying processes using technologies that require the use of high temperatures or abrupt changes that differs from the normal temperature conditions used for optimum growth of these BLAB. Although these bacteria have been shown to have good beneficial properties it is important to know their technological features to be include in to the probiotic product. The aim of this work was evaluate the BALB viability in different thermic conditions. The beneficial strains studied were Lactobacillus reuteri (3 strains), L. gasseri (12), L. rhamnosus (4), L. salivarius (2), L. johnsonii (1), L. paracasei (2) and L. delbrueckii (6). Strains cultures of BLAB in stacionary phase growth were washed twice with buffer solution (PBS pH= 6.5) and later inoculated at 2% in MRS and incubated at different temperatures (37ºC, 42ºC, 50ºC and 55ºC) for 24 hours. The optical density (OD560nm) indicate the growth in a period of time, at 3, 6, 9, 12 and 24 h and growth´s parameters were analized. The results indicate that most of the strains had similar growth at 37 ºC and 42ºC with a 95 % of significance (p-value< α). The strains didn´t growth at 50 ºC and 55 ºC. However, after 9 hours of incubation they could continuous growing at 37ºC during 24 hours more (later).The results obtained support that these BALB can be subjected to extreme thermal processes to be applied in the design of final pharmabiotics formulations.