EFFECT OF PROBIOTIC ADMINISTRATION ON BIOCHEMICAL PARAMETERS AND INTESTINAL MICROBIOTA IN CALORIC RESTRICTION

FABERSANI, E; RUSSO, M; ROSS, R; MEDINA, R; GAUFFIN CANO, P

Mar del Plata

Congreso; - SAIB - 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Effects of caloric restriction diet and Lactobacillus fermentum (Lf) CRL1446 and L. casei (Lc) CRL431 (probioticstrains) administration on metabolic parameters and intestinal microbiota was studied. Balb/c mice were divided intothe following groups: normal diet (ND), caloric restriction diet (CR), CR diet plus Lf and CR plus Lc. CR diet wasadministered during 45 days and strains were given in the dose of 10 cells/mL/day/mouse. Triglyceride (TG), totalcholesterol (TC), glucose and leptin were determined in plasma. Gut microbiota was evaluated by high-throughputsequencing of 16S rRNA gene amplicons. Glucose, TC, TG and leptin levels were decreased in CR groups. Lcadministration increased glucose and leptin levels. Lf decrease TC and leptin levels, showing lower values than in CRgroup. Gut microbiota studies, showed that CR diet increased abundance of Bifidobacterium and Lactobacillus genusand improved Bacteroidetes:Firmicutes ratio. Lc administration increased abundance of Lactobacillus genus.However, Lf administration increased abundance of Bifidobacterium genus and was able to maintain Lactobacillusgenus during time of treatment. Probiotic strains improved metabolic parameters modulating intestinal microbiotadifferently. Lc and Lf increased dominance of certain species reducing biodiversity. L. fermentumexerted abifidogenic effect under CR conditions