Oral administration of Lactobacillus fermentum CRL1446 improves biomarkers of metabolic syndrome in mice fed a high-fat diet supplemented with wheat bran

RUSSO, M.; MARQUEZ, A.; HERRERA, H.; ABEIJON-MUKDSI, C.; SAAVEDRA, L.; HEBERT, E.; GAUFFIN-CANO, P.; MEDINA, R.

Food & Function

Royal Society of Chemistry

Lugar: Cambridge; Año: 2020 vol. 11 p. 3879 - 3894

2042-6496

This work aimed to evaluate the effect of oral administration of probiotic Lactobacillus (L.) fermentumCRL1446, with feruloyl esterase (FE) activity, on metabolic biomarkers and intestinal microbiota of micewith high fat diet-induced Metabolic Syndrome (MS) and supplemented with wheat bran as a source ofesterified ferulic acid. Six-week-old male Swiss albino mice developed the components of MS when fedwith high fat diet supplemented with wheat bran (HFD + WB) for 14 weeks. Positive impact ofL. fermentum CRL1446 administration on these animals was reflected in a decrease in body weight gainand adiposity index compared to the animals that did not receive the probiotic strain. In addition, adecrease in plasma leptin levels, improvement of inflammatory profile, reduction of fatty infiltration inhepatocytes and modification of lipid profile (increased HDL-cholesterol and decreased LDL-cholesteroland triglyceride levels) were observed. On the other hand, L. fermentum CRL1446 reduced fastingglucose and insulin levels, improving the HOMA index in mice with MS. Postprandial glucose levels werealso reduced in the oral glucose tolerance test. Consumption of L. fermentum CRL1446 with HFD + WB(HFD + WB-Lf mice group) had a great impact on host metabolism, modulating intestinal microbiota, withan increase in Bacteroidetes and a decrease in Firmicutes abundance being observed. Increased intestinalFE activity, improved oxidative status and increased abundance of 3-hydroxyphenylpropionic acid andbutyric acid concentration in colonic content, were also demonstrated in HFD + WB-Lf mice. Resultsobtained suggest that supplementation with L. fermentum CRL1446 enhances beneficial effects of a brandiet, attenuating the risk factors associated with MS.