POSTBIOTIC FROM Lactobacillus rhamnosus CRL1505 IMPROVES EMERGENCY MYELOPOIESIS IN CYCLOPHOSPHAMIDE IMMUNOCOMPROMISED MICE

VASILE, BRENDA; IVIR, MAXIMILIANO; GUTIÉRREZ, FLORENCIA; ALVAREZ, SUSANA; SALVA, SUSANA

San Miguel de Tucuman

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Emergency myelopoiesis, replenishment of mature myeloid cells, proliferation and differentiation of stem cells in the bone marrow (BM), are impaired during the chemotherapy-induced myelosuppression. Lactobacillus rhamnosus CRL1505 (Lr1505) diet supplementation is an interesting alternative to improves steady-state myelopoiesis. Although the Lr1505 viability is important, it is possible to stimulate immunity by using the non-viable microorganism. In this work, we evaluated whether Lr1505 and its cell wall (CW1505) was able to improve emergency myelopoiesis induced by TLR4 agonist. Adult Swiss-mice were orally treated with Lr1505 or CW1505 during 10 consecutive days. On day 6, treated and untreated mice received one intraperitoneal dose of cyclophosphamide (Cy 150mg/kg). On day 3 post-Cy injection, mice were challenged intraperitoneal with LPS (10mg/kg weight). The inflammatory challenge induced a significant increase of the number of total, F4/80+ and Gr-1+Ly6G+ cells in the peritoneal cavity, reached a peak at 6h, and returned to the initial values at 24h post-LPS in all groups (p