Differential effects of lactic acid bacteria pptidoglycans on lung epithelial cells and alveolar macrophages.

KOLLING, YANINA; FERRERO, MARIANA; SALVA, SUSANA; BALDI, PABLO; ALVAREZ, SUSANA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Inmunología

We demonstrated that peptidoglycan from Lactobacillus rhamnosus CRL1505 (Pg05) is as effective as viable strain for improving systemic and respiratory humoral immune response against Streptococcus pneumoniae in malnourished mice. These immunomodulatory capacity cannot be extrapolated to peptidoglycans (Pgs) from other strains. The aim of this work was to study the interactions of Pgs obtained from different lactobacilli, with mice lung epithelial cells (LEC) or alveolar macrophages (AM), in order to elucidate the mechanisms involved in the differential effect of Pg05. We compared Pg05 with Pgs from the non-probiotic L. rhamnosus CRL534 (Pg534) and from the probiotic L. plantarum CRL1506 (Pg06). AM or LEC were cultured 24h or 24-48h with different concentrations of Pg05, Pg06 or Pg534, in the absence or presence of S. pneumoniae. In addition, we studied the cytokines production of LEC with conditioned medium from AM pre-stimulated with Pgs. The results showed that 90% of LEC and AM remained viable after the treatments with Pgs. Pg05 (50 ug/mL) and Pg06 were able to differentially modulate the cytokines production in AM and LEC. Pg05 significantly modulated the activation of AM by an increase of IL-1β, KC and IL-10 with a decrease of TNF-α with respect to the other Pgs (p