Lactobacillus casei CRL431 reduces bone marrow histological alterations induced by cyclophosphamide

HERRERA MATÍAS; VILLENA JULIO; SALVA SUSANA; BARBIERI NATALIA; ALVAREZ SUSANA

Tafí del Valle. Tucumán

Jornada; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; 2009

Asociación de Biología de Tucumán

We evaluated the effect of L. casei CRL431 (Lc) administration on bone marrow (BM) histological and cytomorphological changes, induced by cyclophosphamide (CF) in mice. A group of mice was fed Lc for 2 d, this group and untreated control mice (C) received an intraperitoneal injection of CF (150 mg/kg). Before and after CF injection we carried out total and differential cell counts in BM and histological studies. CF administration decreased significantly BM cell numbers in both groups, affecting mainly non-mitotic pool cells. Lc mice showed a lower decrease of BM leukocytes and higher levels of mitotic pool cells when compared with C group. Histological studies showed that CF decreased BM myeloid and lymphoid series (1-3d post-CF). In the C group, eritroblasts, adipocytes and megakaryocytes were the predominat cells. In addition, accumulation of acidophilic material and sinusoids alteration were observed (3-4 d post-CF). The BM architecture was recovered on d 10. In mice treated with Lc the myelo/erythroid ratio was higher than in the C group and myeloid, lymphoid and mature red cells were less affected than in the C group (1-3d post-CF). In the Lc treated mice, the BM architecture was normalized on d 7. According these results, preventive administration of probiotic lactic acid bacteria could be able to reduce BM histological alterations associated with chemotherapy treatments.