Citokine profile induced by probiotics during an infection intestinal and its relationship with the immune response

SALVA SUSANA; VILLENA JULIO; NUÑEZ DE KAIRUZ MARTHA; FONT GRACIELA; ALVAREZ SUSANA

Tafí de Valle. Tucumán

Jornada; XXV Jornadas Científicas de la Asociación de Biología de Tucumán; 2008

Asociación de Biología de Tucumán

Lactobacillus casei CRL 1505 (Lc05) and CRL 1506 (Lc06) can increase the number of IgA+ cells in intestine and peritoneal macrophages bactericidal activity. Aim: to investigate the ability of both strains to stimulate immunity against Salmonella typhimurium (St). Swiss mice received Lc05 or Lc06 (108cells/ day/mouse) for 5d. On d6, mice were infected with St. After challenge liver, spleen and blood cultures; total and differential leukocytes in blood; activity of phagocytic cells in blood and peritoneal cavity; intestinal fluid (IF) and serum anti-St IgA and IgG; IF and serum TNF-α, INF-γ, IL-1, IL-4, IL-6 and IL-10 were determined. Both treatments reduced significantly St counts in liver, spleen and blood. This effect was related to a stimulation of innate immunity, because treated mice showed greater phagocyte activation and increased production of pro-inflammatory cytokines. There was also an improved humoral response against St in treated mice, which correlated with significantly higher levels of IL-4 and IL-10. In addition, levels of IF and serum INF-γ were higher than those of control mice, suggesting that both strains are also capable of stimulating cellular immunity. Administration of Lc05 or Lc06 are able to improve immunity against St. This effect will be related to the profile of cytokines induced, both locally and systemically.