Renutrition with human milk: effects on hemopoiesis and phagocytic cells

GARCÍA DE OLIVA ISABEL; VILLENA JULIO; SALVA SUSANA; AG¡ÜERO GRACIELA

Tafí del Valle. Tucumán

Jornada; XXI Jornadas Científicas de la Asociación de Biología de Tucumán; 2004

Asociación de Biología de Tucumán

We demonstrated that goat milk, improved bactericide activity in malnourished mice. The aim was to study the effects of human milk (HM) on hemopoiesis and innate immune response in malnourished mice, in a comparative way with goat and cow milk (GM and CM). Different groups of malnourished mice were fed with HM, GM and CM for 7 d and 14d. We determined: 1) Percentage of body weight (BW) increase, thymus weight (TW), haematological parameters and traferrin 2) CYtomorphological and T maturation marker by cytochemical studies (Beta-Glucuronidase and Alfa-naphthyl butyrate esterase positive cells) in bone marrow (BM) 3) Peroxidase reaction (Px) in blood leukocytes neutrophils and Nitro Blue Tetrazolium test (NBT) in peritoneal macrophages to study phagocytic activity. Results HM normalized the thymus weight at 7d, and CM and GM at 14d. HM was the best supplement to increase the blood leukocytes number and percentage of cells from mitotics pool in BM. The Px activity and NBT test showed improvement with the different kind of milks. Conclusion. The HM was more efficient to improve the immune system. At 7d thymus reach the normal weight, whereas this effect, was obtained at 14d with GM and CM. Px activity in peripheral blood leukocytes neutrophils, showed a tendency to be higher when the mice received HM than CM or GM.