Blood immune cells subsets in children with post Adenovirus bronchiolitis obliterans and frequent respiratory tract infections

ARGAÑARAZ, NICOLÁS; SALVA, SUSANA; ALBARRACIN, LEONARDO; ALVAREZ, SUSANA; SOLORZONO, M N; CACCIATO, E; GOBBATO, NADIA; VILLENA, JULIO; VALDEZ, JUAN CARLOS; OROSCO, S

San Luis

Congreso; LXXI Reunión Científica Anual de la Sociedad Argentina de Inmunología SAI; 2023

Sociedad Argentina de Inmunología

Post Adenovirus infection Bronchiolitis Obliterans is a pathologic entity characterized by airways fibrosis. In the worldwide are relatively rare but in Latin America appears significantly elevated. These patients present variable course and outcomes, being one of the long-term complications the frequent respiratory tract infections. Unfortunately, the development of immunology or its long-term consequences of post infection bronchiolitis obliterans PIBO by Adenovirus have not been studied in depth. This work aimed to evaluate blood immune cells subsets in children attending to the neumonology service with a history of PIBO by Adenovirus during the first year of life and frequent respiratory tract infections. Five patients with PIBO (8-12 years, 2 males, 3 females) that attended service for respiratory infection symptoms were selected for the study. Blood samples were taken at time of consultation (t1) and after 21-23 days (t2) for flow cytometric studies. Total leucocytes, monocytes and granulocytes counts, and CD45+Annexin+, CD45+HLA-DR+, CD16+CD56+ NK, CD19+ B cells as well as CD3+CD4hi, CD3+CD8+, CD3+CD25+, and CD3+HLA-DR+ T cells were evaluated at t1 and t2. All the patients presented symptoms of respiratory infections at t1. Four children had no symptoms at t2 and patient 1 (P1), despite presenting clinical improvement, still had symptoms. At t1, all the patients had significantly higher (p