In vitro effect of vaginal lactobacilli on the growth and adhesion abilities of uropathogenic Escherichia coli

MARIA CECILIA LECCESE TERRAF; MARIA SILVINA JUAREZ TOTMAS; LUCIE RAOULT; YVES LE LOIR; SERGINE EVEN; MARIA ELENA FÁTIMA NADER

ARCHIVES OF MICROBIOLOGY

SPRINGER

Lugar: Berlin; Año: 2016 p. 1 - 20

0302-8933

Escherichia coli is one of the main causes of uncomplicated urinary tractinfections and responsible of vaginal infections. Lactobacilli can inhibit thispathogen by the production of antimicrobial substances as organic acids,hydrogen peroxide and/or bacteriocins. The aim of this work was to study theeffects of beneficial vaginal lactobacilli on E. coli through in vitro experiments.The inhibitory activity of three vaginal Lactobacillus strains against E. coli was assessed using the agar plate diffusion. Moreover, the effect of Lactobacillus reuteri CRL (Centro de Referencia para Lactobacilos Culture Collection) 1324 on the adhesion and internalization capabilities of E. coli was studied on HeLa cells. Two Lactobacillus strains inhibited the growth of the pathogens by production of organic acids. L. reuteri CRL 1324 reduced the adhesion andinternalization of E. coli 275 into HeLa cells. The results obtained suggest that L. reuteri CRL 1324 can be considered as a probiotic candidate for further in vivo studies for the prevention or treatment of urinary tract infections caused by E. coli.