Genomic Insights into Enterococcus faecalis Strains Isolated from Meat.

BONACINA J; SUAREZ, N; SAAVEDRA L

Bahia Blanca

Congreso; VI Congreso Argentino de Bioinformática y Biología Computacional (CAB2C); 2015

AB2C2

The aim of this work was to perform a comparision, at the genomic level, of the meat isolates Enterococcus faecalis 2924 (Ef 2924) and Enterococcus faecalis 19116 (Ef 19116) from turkey and pork respectively, with the first vancomycin-resistant enterococcal isolate in the U.S, Enterococcus faecalis V583 (Ef V583). For this purpose, a functional annotation and metabolic reconstruction was performed with the Rapid Annotation Subsystem Technology (RAST) server. In this regard it was observed that Ef 19116 and Ef 2924 had the same subsystem coverage (Fig.1), with 1338 non hypothetical coding sequences (cds) assigned to subsystems, each; meanwhile Ef V583, with a higher number of CDS annotated, had 1401. In both strains of meat origin, 7 pegs for menaquinone and phylloquinone biosynthesis (complete set), 5 involved in the mevalonate branch of isoprenoid biosynthesis and 8 for inositol catabolism, were assigned to a subsystem; this was not observed for Ef V583. On the other hand the clinical isolate 5 cds coding for sortases, 21 pegs involved in hyaluronate utilization, 8 in butanol biosynthesis and 3 in acetyl-CoA fermentation to butyrate, that were not present in Ef 19116 and Ef 2924. In both strains of meat origin, 2 pegs for tetracycline resistance related to ribosome protection and 4 associated with the CRISPR system were detected. These features were not identified in Ef V583. On the other hand the clinical isolate had 5 genes belonging to the Streptococcus pneumoniae vancomycin tolerance locus and phage-related proteins.Next, a search for antibiotic resistance genes in the enterococcal genomes was carried out with the ARG-ANNOT (Antibiotic Resistance Gene-ANNOTation) tool capable of detecting existing and putative new antibiotic resistance (AR) genes blasting the corresponding sequences against an up to date database. Interestingly, several van genes were localized in Ef 2924 genome, despite not being detected using the RAST tool. Furthermore, genes related to class glycopeptides (GLY), macrolide-lincosamide-streptogramin (MLS), tetracyclines (tet), aminoglycosides (AGly) trimethoprim (Tmt) were also distinguished (Table 1).