INVESTIGADORES
SAAVEDRA Maria Lucila
artículos
Título:
Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells
Autor/es:
KANMANI, PAULRAJ; ALBARRACIN, LEONARDO; KOBAYASHI, HISAKAZU; HEBERT, ELVIRA MARIA; SAAVEDRA, LUCILA; KOMATSU, RYOYA; GATICA, BRIAN; MIYAZAKI, AYAKO; IKEDA-OHTSUBO, WAKAKO; SUDA, YOSHIHITO; ASO, HISASHI; EGUSA, SHINTARO; MISHIMA, TAKASHI; SALAS-BURGOS, ALEXIS; TAKAHASHI, HIDEKI; VILLENA, JULIO; KITAZAWA, HARUKI
Revista:
Frontiers in Immunology
Editorial:
Frontiers Media S.A.
Referencias:
Año: 2018 vol. 9
Resumen:
In lactic acid bacteria, the synthesis of exopolysaccharides (EPS) has been associatedwith some favorable technological properties as well as health-promoting benefits.Research works have shown the potential of EPS produced by lactobacilli todifferentially modulate immune responses. However, most studies were performed inimmune cells and few works have concentrated in the immunomodulatory activitiesof EPS in non-immune cells such as intestinal epithelial cells. In addition, thecellular and molecular mechanisms involved in the immunoregulatory effects of EPShave not been studied in detail. In this work, we have performed a genomiccharacterization of Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and evaluatedthe immunomodulatory and antiviral properties of its acidic (APS) and neutral (NPS)EPS in porcine intestinal epithelial (PIE) cells. Whole genome sequencing allowedthe analysis of the general features of L. delbrueckii TUA4408L genome as wellas the characterization of its EPS genes. A typical EPS gene cluster was foundin the TUA4408L genome consisting in five highly conserved genes epsA-E, anda variable region, which includes the genes for the polymerase wzy, the flippasewzx, and seven glycosyltransferases. In addition, we demonstrated here for thefirst time that L. delbrueckii TUA4408L and its EPS are able to improve theresistance of PIE cells against rotavirus infection by reducing viral replication and regulating inflammatory response. Moreover, studies in PIE cells demonstrated that theTUA4408L strain and its EPS differentially modulate the antiviral innate immune responsetriggered by the activation of Toll-like receptor 3 (TLR3). L. delbrueckii TUA4408L and itsEPS are capable of increasing the activation of interferon regulatory factor (IRF)-3 andnuclear factor kB (NF-kB) signaling pathways leading to an improved expression of theantiviral factors interferon (IFN)-b, Myxovirus resistance gene A (MxA) and RNaseL.