Oral administration of Lactobacillus fermentum CRL1446 improves biomarkers of metabolic syndrome in mice fed a high-fat diet supplemented with wheat bran

RUSSO, M.; MARQUEZ, A.; HERRERA, H.; ABEIJON-MUKDSI, C.; SAAVEDRA, L.; HEBERT, E.; GAUFFIN-CANO, P.; MEDINA, R.

Food & Function

Royal Society of Chemistry

Año: 2020 vol. 11 p. 3879 - 3894

2042-6496

This work aimed to evaluate the effect of oral administration of probiotic Lactobacillus (L.)fermentum CRL1446, with feruloyl esterase (FE) activity, on metabolic biomarkers and intestinalmicrobiota of mice with high fat diet-induced Metabolic Syndrome (MS) and supplemented withwheat bran as a source of esterified ferulic acid. Six-week-old male Swiss albino mice developedthe components of MS when fed with high fat diet supplemented with wheat bran (HFD+WB) for14 weeks. Positive impact of L. fermentum CRL1446 administration on these animals was reflectedin a decrease in body weight gain and adiposity index compared to the animals that did not receivethe probiotic strain. In addition, a decrease in plasma leptin levels, improvement of inflammatoryprofile, reduction of fatty infiltration in hepatocytes and modification of lipid profile (increasedHDL-cholesterol and decreased LDL-cholesterol and triglyceride levels) were observed. On theother hand, L. fermentum CRL1446 reduced fasting glucose and insulin levels, improving theHOMA index in mice with MS. Postprandial glucose levels were also reduced in the oral glucosetolerance test. Consumption of L. fermentum CRL1446 with HFD+WB (HFD+WB-Lf mice group)had a great impact on host metabolism, modulating intestinal microbiota, with an increase inBacteroidetes and a decrease in Firmicutes abundance being observed. Increased intestinal FEactivity, improved oxidative status and increased abundance of 3-hydroxyphenylpropionic acid andbutyric acid concentration in colonic content, were also demonstrated in HFD+WB-Lf mice.Results obtained suggest that supplementation with L. fermentum CRL1446 enhances beneficialeffects of a bran diet, attenuating the risk factors associated with MS.