SAAVEDRA Maria Lucila
Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken ? relevance of in vitro and ex vivo methods for strains characterization
BABOT JAIME; ARGAÑARAZ-MARTINEZ ELOY; SAAVEDRA L; MARIA CRISTINA APELLA; PEREZ CHAIA ADRIANA
RESEARCH IN VETERINARY SCIENCE
ELSEVIER SCI LTD
Lugar: Amsterdam; Año: 2014
Based on the natural benefits of the indigenous microbiota, lactic acid bacteria (LAB) from poultry originwere isolated from hens and broilers intestine, and their probiotic potential was further studied. The toleranceto digestion, adhesion, capture of a mannose-binding lectin, absence of virulent factors and antibioticresistances were studied. Different in vitro and ex vivo assays were performed to select tolerantand adherent strains because standardized protocols have not been defined. Fourteen strains highly tolerantto gastrointestinal digestion were genetically identified. Hydrophobic surfaces were not requiredfor the bacterial adhesion and only nine strains adhered ex vivo to the intestinal mucosa. Three strainscaptured a lectin of the same specificity of Type-1 fimbriae. Virulence factors were absent but some strainsevidenced multiple antibiotic resistances. These results provide bases for a future standardization of methodsfor the selection of probiotic strains intended to reinforce the microbiota of newly hatched chickens.