Antifungal activity of a metabolite purifed from terminalia triflora

CORONEL, R.S.; ANGOS, A.; SGARIGILA, M. A.; SAMPIETRO D. A.; SOBERÓN , J.R.

Tafí del Vale - Tucumán

Congreso; XXXV Annual Scientific Meeting of Tucuman Biology Society; 2018

Tucuman Biology Society

Current human fungal infection therapies due to Candida albicans are limited by fungistatic action, toxicity or development of resistance. Terminalia triflora (Tt) is a species with antifungal folk uses, and a potential source of natural antifungal compounds. Tt leaves were extracted with ethanol, and successively fractionated with solvents in an increased polarity order, chromatography, solid phase extraction and HPLC to yield an antifungal compound (P1). P1 antifungal activity against C. albicans (ATCC10231) was tested. Minimal inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) were obtained through microdilution and cell viability assay for P1 alone and in combination with fluconazole (FLU). P1 (100 µg/mL) was active against C. albicans only in combination with FLU (0,5 µg/mL). The FICI=0.258 indicated synergistic effect between P1 and FLU. P1 + FLU resulted in a fungicidal combination. The results give support to the ethnomedicinal applications described for Tt.