Isolation and identification of an antifungal metabolite from Tessaria dodoneifolia

SOBERÓN, J.R.; SGARIGLIA, M.A.; AGUILAR, F.A.; CARABAJAL TORREZ, J.A.; SAMPIETRO, D.A.; VATTUONE, M. A.

Tafí del Vale - Tucumán

Congreso; XXXIV Annual Scientific Meeting of Tucuman Biology Society; 2017

Tucuman Biology Society

Most fungal infections in humans are due to Candida albicans strains. Tessaria dodoneifolia (Td) is a species with antifungal folk uses, being potential source of natural antifungal compounds. An ethanolic extract was obtained from Td leaves, and fractionated by with solvents in an increased polarity order, adsorption chromatography and HPLC, to yield an antifungal compound identified as Naringenin (NAR) through NMR and polarimetric assays. Antifungal activity against C. albicans (ATCC10231) was evaluated to obtain Minimal inhibitory concentrations (MIC) and fractional inhibitory concentration index (FICI) for NAR and fluconazole (FLU) (alone and combined) by microdilution assay. The MIC for NAR (without FLU) was 40 mg/L and the FICI=0.258, which indicated synergistic effect between NAR and FLU. NAR + FLU resulted in a fungicidal combination. The results give support to the ethnomedicinal applications described for Td.