Antifungal activity of Anagallisin C against Candida albicans

SOBERÓN, J.R. ; SGARIGILA, M.A.; SORUCO, E.M.; SAMPIETRO D. A.; VATTUONE M.A.

Tafí del Vale - Tucumán

Congreso; XXXIII Annual Scientific Meeting of Tucuman Biology Society; 2016

Tucuman Biology Society

C. albicans is an opportunistic pathogenic yeast responsible for infections that could affect various organs. The drug for most often used against candidiasis is fluconazole (FLU), an azole drug with fungistatic action, an aspect that favors the development of drug resistance. To overcome drug resistance a combination of drugs is suggested. Anagallisin C (AnC) is the main secondary metabolite isolated from Anagallis arvensis L aerial parts and could be an alternative for fungal infection treatments, alone or in combination with FLU. AnC antifungal activity against C. albicans (ATCC10231) was preliminarily evaluated by disk diffusion assay with and without FLU (6 mg/L) to statistically analyze synergistic, indifferent or antagonistic effects. Minimal inhibitory concentrations (MICs) and fractional inhibitory concentration (FIC) of AnC and FLU (alone and combined) were obtained by microdilution assay. The interpretation of FIC was: ≤0.5, synergistic effect; >0.5 to< 4.0, indifferent effect; ≥4.0, antagonistic effect. There were significant differences (p