Inhibition of Alternaria alternata) growth by Raphanus sativus L. (nabón) extracts. separation of fungitoxic compounds

SGROI, N.A.; SELIS, A.N.; SGARIGLIA, M.A.; SOBERÓN, J.R.; QUIROGA, E.N.; VATTUONE, M.A.

Tucumán - Argentina

Congreso; XXV Annual Scientific Meeting of Tucuman Biology Society; 2008

Tucuman Biology Society (publicado en Bio Cell 33 (2))

Aqueous (infusion and decoction) and ethanolic (tincture) extracts (10% w/v)  were prepared from dry and ground siliques. The fungal inhibitory capacity of the extracts was assayed by radial growth inhibition in glucose-potato-agar (GPA) plates. The percentage of inhibition of A. alternata  growth produced by 15 mg extracted material/mL (EM/mL) was 7%, 32% and 41% for infusion, decoction and tincture, respectively. The yield of the extractions was 8,10; 2,25; 0,45 mg phenolic compounds/mL (FC/mL) for decoction, infusion and tincture, respectively. Extraction of dried tinctures with solvents of increasing polarity (ethyl ether, dichloromethane and methanol) allowed to detect that the dichloromethane extract was the most active. Several TLC analysis allowed the detection of terpenoids and essencial oils. Experimentation is orientated to isolate the compounds responsible of the antifungal activity.