Analysis of genotoxicity of isolated compounds from Tripodanthus acutifolius leaves

SOBERÓN, J.R.; SGARIGLIA, M.A.; SAMPIETRO, D.A.; QUIROGA, E.N.; VATTUONE, M.A.

Tucumán - Argentina

Congreso; XXV Annual Scientific Meeting of Tucuman Biology Society; 2008

Tucuman Biology Society (publicado en Bio Cell 33 (2))

Plant extracts may have significant biological activities (antiinflammatory, antibacterial or antioxidant), but also may exhibit genotoxicity (genomic toxicity). Purification of biactive compounds may diminish or avoid that toxicity. Infusion from T. acutifolius leaves has antibacterial, antioxidant and antiinflamatory properties. Five phenolic compounds, partially responsible for those activities, were purified and characterized from infusion. The genotoxic activity of all samples was studied by using Bacillus subtilis rec assay, which measures the differential growth of DNA repair proficient and deficient B. subtilis strains (called rec+ and rec – respectivelly), and the results were expressed as S-probit values. K2Cr2O7 (strong genotoxic) and Kanamycin (non genotoxic) were used as reference substances. The results showed that infusion has strong genotoxic response (S-probit=0.8), while the isolated compounds: rutin, nicotiflorin, hyperoside, isoquercitrin and tripodanthuside have non genotoxic responses (S-probit<0.2). The results show that toxicity of a raw extract could be avoided after purification of the bioactive constituents.