Free radical scavenging activity of purified compounds from Tripodanthus acutifolius (Ruiz & Pavón) Van Tieghem

SOBERÓN, J.R.; SGARIGLIA, MA.; SAMPIETRO, D.A.; QUIROGA E. N.; VATTUONE, M.A.

Tucumán - Argentina

Congreso; XXIV Annual Scientific Meeting of Tucuman Biology Society; 2007

Tucuman Biology Society (publicado en Bio Cell 32 (2))

Free radicals are high reactive molecules responsible of various diseases. The health impact of uncontrolled free radicals proliferation and the known toxicity of synthetic antioxidants lead the researching of alternative free radical scavenging molecules. Five antioxidant compounds were isolated and identified from T. acutifolius leaves infusion. Purification was achieved after extraction with increasing polarity solvents, column chromatography through sephadex LH-20, and reverse phase HPLC. Four compounds were identified as glycoflavonoids: rutin, nicotiflorin, hyperoside and isoquercitrin. The other one was a novel compound: 4-(3`,4`-dihydroxy)phenil-2-â-D-glucofuranosylbutane (DHGB). DHGB was more active by DPPH and lipid peroxidation inhibition assays [effective concentration 50 (EC50) = 3,4 and 120 ppm respectively] than the other compounds. Hyperoside and isoquercitrin were more effective hydroxyl radical scavengers (EC50 = 100 ppm) than the other compounds. Flavonoids and DHGB´s phenolic groups could be responsible of the free radical scavenging activities.