Antifungal activity of 4-hydroxy-3-(3-methyl-2-butenyl)acetophenone against Candida albicans

SOBERÓN, J.R.; LIZARRAGA, E.F.; SGARIGILA, M. A.; SAMPIETRO, D.A.; BEN ALTABEF, A.; CATALÁN, C.A.N.; VATTUONE, M.A.

Horco Molle - Yerba Buena - Tucumán - Argentina

Congreso; XXXI ANNUAL SCIENTIFIC MEETING - TUCUMAN BIOLOGY ASSOCIATION; 2014

TUCUMAN BIOLOGY ASSOCIATION

C. albicans is opportunistic fungal pathogen, and a common cause of invasive fungal infections in humans, producing infections that can involve any organ. The most used drug for candidiasis is fluconazole (FLU), an azole drug with fungistatic action, an aspect that favors the development of drug resistance. To overcome drug resistance a combination of drugs is suggested to be used. The main secondary metabolite of Senecio nutans Sch. Bip (Asteraceae) aerial parts is 4-hydroxy-3-(3-methyl-2-butenil)acetophenone (4HMBA), preliminary reported as antifungal, which could be an alternative for fungal infections treatments, alone or in combination with FLU. 4HMBA antifungal activity against C. albicans (ATCC10231) was preliminary evaluated by disk diffusion assay with and without FLU (6 mg/L) to statistically analyze synergistic, additive or antagonist effects. Minimal inhibitory concentrations (MICs) and fractional inhibitory concentration (FIC) of 4HMBA and FLU (alone and combined) were obtained by microdilution assay. The interpretation of FIC was: ≤0.5, synergistic effect; >0.5 to< 4.0, additive effect; ≥4.0, antagonic effect. There were significant differences (p