Caesalpinia paraguarienses extract: its potencial application as natural antiinflammatory

SGARIGLIA, M. A.; SOBERÓN, J. R.; SAMPIETRO, D. A.; QUIROGA, E. N.; VATTUONE, M. A.

Rosario (Argentina)

Congreso; XLII Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2006

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

Hyaluronic Acid is responsible for structural and functional integrity of articular cartilage. When this tissue is target of chronic inflamatory pathologies, e.g. Osteoarthritis, Hyaluronidases (HYAL) are activated and generate low weight molecular fragments. These promote the release of pro-inflamatory cytokines that activates other degrading enzymes, contributing to the progressof the disease. C. paraguariensis ritidome infusion is rich in polyphenols (pf); these compounds are associated to diverse pharmacologic effects, among them antiinflammatory. The research is focused on the inhibitory activity of this extract on HYAL, and its relation with tannins, other pf or both compounds. The Infusion (1) was lyophilized ad sequentially extracted with: Ether (2), Chloroform (3), Methanol (4). BSA was added to etract (4), and the supernatant (5) was separated by centrifugation. Pf were determined by Price y Butler Method. The inhibitory effect on HYAL was evaluated by a colorimetric method based on Morgan-Elson  Reaction. Reference substances: Aspirin (A) and Gallic acid (GA). The HYAL inhibition percentage obtained were: 75,1; 0,6; 14,9; 73,9; 71,3; 34,3; 10,2; for extracts (1), (2), (3), (4), (5), (A) and (GA), respectively. It is concluded that (1) display inhibitory effect of HYAL and that this activity is associated to pf or other compounds different from tannins.