Isolation of antioxidants from Tripodanthus acutifolius (Ruiz & Pavón) Van Tieghem leaves

SOBERÓN, J. R.; SGARIGLIA, M. A.; SAMPIETRO, D. A.; QUIROGA, E. N.; VATTUONE, M. A.

Rosario (Argentina)

Congreso; XLII Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2006

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

Introduction: Hydroxil radicals (OH) are oxygen species generated in reactions with iron or copper ions. Proteins and lipids are target of OH attack. Within the cell nucleus HO damages deoxyribose and nitrogen bases of DNA, causing heritable mutations and cancer. Many diseases are attributed to the deleterious effects of HO attack. Objective: to study the OH scavenging activity of infusion from T. acutifolius leaves and to isolate and identify the responsible substances. Methods: deoxyribose assay was performed to asses the site specific (SS) and non-site specific (NSS) scavenging activities of T. acutifolius infusion. The isolation of the antioxidant substances was performed by Silica gel column chromatography (CC), Sephadex LH 20 CC, and preparative Silica gel TLC plates. The identification of eluted compounds was achieved by comparison of their UV-Visible spectra with standards. Results: T. acutifolius infusion showed HO scavenging activity by SS and NSS assays (EC50 = 55.2 and 213 ppm respectively). The bioactive compounds were identified as glycoflavonoids: rutin, iso-quercitrin and hyperoside. Conclusion: Flavonoids have the ability to protect biosystems against HO attack. Those flavonoids may act as direct HO scavengersor iron chelating substances. The antiinflamatory effect of T. acutifolius infusion could be partially attributed to the identified flavonoids.