Genotoxic analysis of Caesalpinia paraguariensis (D. PARODI) BURKART extracts

SGARIGLIA, M.A.; SOBERÓN, J.R.; SAMPIETRO, D.A.; QUIROGA E.N.; VATTUONE, MA.

Tucumán - Argentina

Congreso; XXIII Annual Scientific Meeting of Tucuman Biology Society; 2006

Tucuman Biology Society (publicado en Bio Cell 31 (2))

The Argentinean folkloric medicine uses extracts from plants (infusions, decoctions and tinctures), that are constituted by compounds of different chemical nature and biological properties. Commonly, the use of these preparations in order to cure is not thought as bearer of some toxicity. We investigate the C. paraguariensis extracts, appreciated species for its vulnerary properties, as for its capacity to cause damage to DNA molecule, its genotoxicity. The employed method is known as "rec- assay", with isogenic Bacillus subtilis strains. This is based on the relative difference of survival of a DNA repair-recombination proficient strain (H17: rec+) and its deficient strain (M45: rec-). This is represented for two curves that enclose an area, calculated by Probit transformation (S-probit). Infusion and Decoction are not genotoxic (S-probit < - 0.123), Tincture is strongly genotoxic (S-probit >0.593). K2Cr2O7 Kanamicine and DMSO were used as strong genotoxic, non genotoxic and reverse effect drugs, respectively. The tincture is not advisable as medicinal.