Natural antioxidants isolated from Tripodanthus acutifolius (Ruiz & Pavón) Van Tieghem

SOBERÓN, J.R.; SGARIGLIA, MA.; SAMPIETRO, D.A.; QUIROGA E. N.; VATTUONE, M.A.

Tucumán - Argentina

Congreso; XXIII Annual Scientific Meeting of Tucuman Biology Society; 2006

Tucuman Biology Society (publicado en Bio Cell 31 (2))

Free radicals are implicated in several health diseases, such as Parkinson's disease, Alzheimer disease, diabetes, cancer, etc. Our previous reports showed that the infusion prepared from T. acutifolius leaves has free radical scavenging activity. The aim of this study was to perform a biodirected isolation and identification of the antioxidant substances from T. acutifolius infusion. Lyophilized Infusion was extracted with solvents of increasing polarity. Methanolic fraction was separated by Silica Gel column chromatography and Sephadex LH20. Final purification was achieved by preparative TLC developed with ethyl acetate/methanol/water (1 00: 13.5: 10 vlv) as mobile phase. During development of the purification method all steps were monitored by qualitative TLC, and different staining procedures (Vainillin/H2SO4, NP/PEG, and DPPH for antioxidant activity). Rutin, Iso-quercitrin and Hyperoside were isolated and identified by comparison of their UV spectra and chromatographic profiles with those from pure standards. These Glycoflavonoids are responsibles of T. acutifolius infusion antioxidant activity.