Medicinal properties of Caesalpinia paraguariensis bark

SGARIGLIA M.A.; SOBERÓN, J.R.; HONORÉ, S.M.; ANDINA, M.L.; SAMPIETRO, D.A.; SÁNCHEZ, S.S.; VATTUONE, M.A.

Hyderabad

Congreso; International Conference and Exhibition on Traditional & Alternative Medicine; 2013

OMICS group

Caesalpinia paraguariensis (D. Parodi) Burkart is a native species from Argentine, commonly named ?Guayacán?. Bark is used as infusion or additive for ?mate? (a beverage traditional in Argentine), mainly to ameliorate hyperglycemia and hypercholesterolemia due to type 2 diabetes. In this work we assayed the anti-hyperglycemic activity of C. paraguariensis bark infusion (CPBI) estimating the daily dose intakes for a human per Kg body weight (D1: 8 mg/Kg bw), on female Wistar: Fcen/ICBME-HI rats, at 10 weeks old, applied a statistical model randomized block design. Oral sucrose tolerance test was carried out measuring the fasting glycemia, then sucrose (2 g/Kg bw) was administered by gavages, then postprandial glycemia was measured at 0, 15, 30, 45, 60 and 120 minutes. D3 (D1x3), D5 (D1 x5) and Glimepiride also were assayed, last was the positive control. Phytochemical screening of CPBI and quantification of bioactive components previously identified own, which were carried out by analytical chromatographic methods. Doses-response graphs for D1, D3 and D5 show that there are significant differences (ρ > 0.05), respect to control group (C) at T15 and T30 min, such differences were increasingly greater with D3 and D5. The phytochemical screening showed that CPBI contents mainly phenolics components, from several of these have already been structurally identified as ellagic derivatives, which were antioxidant and anti-inflammatory. The rate (% w/w) of components in CPBI were: gallic acid (0.004), 3-O-methylellagic (0,084), 3,3?-di-O-methylellagic (0.077), ellagic acid (0.135), 3,3?-di-O-methylellagic-4-O-β-D-xylopyranoside (0.100). These components were not toxic at amount present in CPBI (D1, D3 and D5).