Combinated antifungal activity of tripodantoside and Amphotericin B against Candida species yeasts

SOBERÓN, J.R.; SGARIGLIA, M.A.; SAMPIETRO, D.A.; VATTUONE, M.A.

Tafí del Valle, Tucumán

Congreso; XXVIII Annual Scientific Meeting of Tucuman Biology Society; 2011

Tucuman Biology Society

Fungal infections caused by yeast pathogens are common in immuno-compromised patients. Candida spp. represents the major group of yeast recovered from these patients. The limited number of available antifungal drugs (azoles, polyenes and pyrimidine analogues), their toxicities and resistance makes necessary to find new antifungal drugs. The main constituent of Tripodanthus acutifolius (Ruiz & Pavon) van Thieghem leaves is tripodantoside (TP), a phenylbutanoid with no data on its antifungal effects. The antifungal activities of TP and amphotericin B (AMB), both individually and combined were assayed against C. albicans (ATCC10231) and C. tropicalis (ATCC750) using broth microdilution method. TP showed inhibitory effects against both strains at 20 μg . mL-1. The association of TP+AMB resulted in additive effects against both strains when combined assays were performed. The combination of TP+AMB could diminish the AMB therapeutic doses and the toxicity associated with the use of AMB. The antifungal activity of TP support deep studies on its action modes.