Antifungal activity of Zuccagnia punctata: comparison with commercial fungicides

JIMENEZ, C.M.; SAMPIETRO, D.A.; SGARIGLIA, M.A.; SOBERÓN, J.R.; VATTUONE, M.A.

Tafí del Valle, Tucumán

Congreso; XXVIII Annual Scientific Meeting of Tucuman Biology Society; 2011

Tucuman Biology Society

Argentina is an important cereal producer. Diseases restrict the grain yields, especially those caused by Fusarium species. Commercial fungicides used for their control have limited efficiency and affect the environment. The aim of this work was to compare the fungicidal and fungistatic activity of an ether fraction (EF) from the tincture of Z. puntacta with commercial fungicides: pyraclostrobin (Py) and epoxiconazole (Ep). The dry material extracted by tincture of Z. punctata was partitioned with diethyl ether. The antifungal activity of the EF (5-250 µg de ME/ml) and the fungicides (0,5-50 µg/ml) were assayed by the microdilution method on F. thapsinum (Ft), F. verticillioides (Fv), F. graminearum sensu stricto (Fse), F.subglutinans (Fs) y F. bothii (Fb). Minimum fungicidal concentration (MFC), minimum inhibitory concentration (MIC), MFC/MIC, and inhibitory concentration 50 (IC50) were determined. IC50s indicated than EF (19-27 μg/ml) produced a similar inhibition on all strains. IC50s of Py and Ep depended from the tested fungus and the most sensitive was Fse (2,4 and 2 μg/ml, respectively). MFCs (100 μg/ml for Fv and Fs; 250 μg/ml for Fb and Ft and 50 μg/ml for Fse) of EF coincided with their MICs, indicating that the observed inhibition is due to a fungicidal action.