Antiinflammatory activity and identification of isolated molecules from Caesalpinia paraguariensis (D. Parodi) Burk

SGARIGILA, M. A.; SOBERÓN, J. R.; SAMPIETRO, D. A.; VATTUONE, M. A.

Tafí del Valle - Tucumán

Congreso; XXVII Annual Scientific Meeting of Tucuman Biology Society; 2010

Tucuman Biology Society

Previous reports showed the anti-inflammatory activi ty of C. paraguariensis bark infusio n by Hyaluronidas e inhibition assay. AIF (anti-inflammatory fraction) was obtained by bio-guided fractionation of the extract. The aims of this stud y were to isolate bioactive constituent(s), to assay enzymatic inhibition , and to structurally identify the bioacti ve molecules. AIF was analyzed by RPHPLC and its components were isolated and purified. Hyaluronidase inhibition assay was carried out by Reiss ig's method . Identification was achieved by spectrometric \tecqniques (UV-Vis, MS-ESI and 'H-RMN). Two eluates were isolated from AIF (M25 and M29), which showed inhibitory concentration 50% (IC50 at ug/ml) : M25 1.5±0.1 and M29 2.3±0. 1. Spectral analysis allowed us to identify them. M25=ellagic acid: MS-ESI: [M-Hrmassl charge (m/z) 301.14 ; Exact mass: 302 .1480 atomic mass unit s (u) . Elemental composition: C'4HP g' 'H-NMR in DMSO-d6 sn (ppm) : Hs.s' 7.51 (s, 2H). UV spect rum: AmaX (MeOH) (nm): 255.5 , 358.0. M29= 3-0-Methyl ellagic acid : MS-ESI: [M-Hrm/z 315.0142. Exact mass : 316.0141 u. Elemental Composition: C'SHp g' 'H-RMN in DMSO-d6 sn (ppm): Hs 7.51 (s, IH), Hs' 7.48 (s, IH), 3-0CH] 4.06 (s, 3H). UV spectrum: AmaX (MeOH) (nm) : 249.9, 346sh, 367.2.