PRELIMINARY STRUCTURAL ANALYSIS OF ANTIFUNGAL METABOLITES OF LEAF ALCOHOLIC EXTRACT FROM PHORADENDRON LIGA

SELIS DE ORSI, A.N.; SGROI, N.A.; SGARIGILA, M. A.; SOBERÓN, J.R.; QUIROGA, E.N.; VATTUONE, M.A.

Tafí del Valle - Tucumán

Congreso; XXVI Annual Scientific Meeting of Tucuman Biology Society; 2009

Tucuman Biology Society

Citrus (lemons) are attacked by fungi which produce large losses in post-harvest. It is possible to use extracts from regional plants as an antifungal treatment. In previous works the antifungal activity of alcoholic extract from Ph. liga against lemon pathogenic fungi was reported. This study describes preliminary analysis to determine the chemical composition of purified fractions obtained by bioguided fractionation. The alcoholic extract was fractionated with three different solvents (ethyl ether, dichlorometane and methanol). The dichlorometane fraction was found to be more effective against Penicillum digitaturm by bioautography. Then, this fraction was fractionated by Sephadex LH20/ methanol. Sub-fractions obtained (G1-G5) were analyzed by TLC (Silica gel 60 F254 plates) and Bioautography. G2 and G4 were the most active. G2 showed three spots and these suggest: G21 diglycosylated phenolic compounds, G22 phenolic acid esters and G23 non-glycosylated phenolic compounds. G4 shows a phenolic compound spot. Additional analysis techniques are needed to confirm these results.