DESIGN OF PROBIOTIC CALF FORMULAS BY LOW COST CULTURE MEDIA WITH DIFFERENT SUGAR SOURCES TO OPTIMIZE LIVE BACTERIAL BIOMASS

MALDONADO, NATALIA C; CARRASCO, NATALIA ; NADER-MACÍAS, MARÍA E FÁTIMA

CABA

Congreso; LIII REUNION ANUAL DE LA ASOCIACION ARGENTINA DE FARMACOLOGIA EXPERIMENTA; 2021

Probiotic formulas for calf containing lactic bacteria (LB) strains must contain high numbers of live microorganisms to exert the beneficial effect. Previously, our research group designed a formula in gelatine capsules containing dry bacteria. LB are nutritional exigent microorganisms, requiring complex culture media to grow, which are very expensive. Increasing then the cost of production. Then, looking for cheaper culture media for industrial applications is highly important. The objective of this work is to study different sugars combinations in a base media to reduce the cost of the biomass obtention, growing the LB at two temperatures. The probiotic strains assayed were: Lactobacillus johnsonii CRL1693, Ligilactobacillus murinus CRL1695, Limosilactobacillus mucosae CRL1696 and Ligilactobacillus salivarius CRL1702. Different media (51) were designed with: Glucose (G), Fructose (F), Molasses (M), Pectin (P), Lactose (L) and Corn syrup (CS). The growth was evaluated by the optical density (OD560nm) in microplates during 24 h at 32 and 37°C, calculating growth parameters. The CRL1693 strains showed significant differences in the growth and Lag phase length (LPL) at the two temperatures. The better growth media resulted with G, L and CS; and combinations of M with G, L and F. CRL 1696 grew in few culture media at both temperatures, and the LPL and OD did not show significant differences, except with a lower growth rate at 30°C. The best combinations at 37°C were: L with G, P and CS. CRL1695 grew in most of the media designed at the two temperatures showing no significant difference. At 37 °C CRL 1702 growth in G, M and L, and in M combined with G, F and in most of the media at the two temperatures, with no significant differences in LPL, but with a higher growth rate at 37 °C. At 32 and 37C, this strain showed good growth in G or M combined with G. The results indicate that the behaviour of the strains is dependent of each strain, even though all the strains assayed are able to grow in the culture media designed with low-cost sugar sources, such as Molasses and Corn syrup for their scaling up for industrial applications.