DESIGN OF A BENEFICIAL PRODUCT 1 FOR NEWBORN CALVES 2 BY COMBINING LACTOBACILLI, MINERALS AND VITAMINS

MALDONADO NC; SILVA DE RUIZ C; NADER ME

PREPARATIVE BIOCHEMISTRY AND BIOTECHNOLOGY

TAYLOR & FRANCIS INC

Lugar: Philadelphia; Año: 2015

1082-6068

Diarrhea is one of the most frequent diseases affecting newborn calves in intensive systems. Several strategies were proposed to protect and improve health, as probiotics. This work was directed to design a product containing freeze dried bacteria, vitamins and minerals. Also, to optimize conditions with lyoprotectors, to combine strains and add vitamins, minerals and inulin to the product. The lyoprotectors were: milk, milk-whey and lactose and products were stored for six month at 4°C. Combined bacteria were freeze-dried in milk and the final products added with minerals, vitamins and inulin. The viable cells were determined by the plate count assay and antibiotic profiles to differentiate strains. Lactobacillus johnsonii CRL1693, L. murinus CRL1695, L. mucosae CRL1696, L. salivarius CRL1702, L. amylovorus CRL1697 and Enterococcus faecium CRL1703 were evaluated. The optimal conditions were different for each strain. Milk and milk-whey maintained the viability during the process and storage after six month for most of the strains, except for L.johnsonii. Lactose did not improve cell¬?s recovery. L. murinus was viable for six months in all the conditions, with similar results in enterococci. In strains combined before freeze-dried, the viability decreased deeply, showing that one-step process with bacteria mixtures, vitamins and minerals was not adequate. Freeze?dried resistance depends on each strain, and must be lyophilized individually.