ADHESION TO THE INTESTINAL MUCOSA IS NOT NECESSARY FOR Lactobacillus rhamnosus CRL1505 TO EXERT ITS IMMUNOMODULATORY ACTIVITIES IN LOCAL AND DISTAL MUCOSAL SITES

TOMOTSUNE, KAE; FERNANDA RAYA-TONETTI; ISLAM, MD AMINUL; LEONARDO ALBARRACÍN; MIZUNO, HIROYA; RAMIRO ORTIZ MOYANO; SUSANA SALVA; SUSANA ALVAREZ; HIDEKI TAKAHASHI; HARUKI KITAZAWA; JULIO VILLENA

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Both, viable and non-viable orally administered Lactobacillus rhamnosus CRL1505 modulate immunity in local (intestine) and distal (respiratory) mucosal sites. Then, intestinal adhesion and colonization would not be necessary for the immunomodulatory effects of this immunobiotic strain. In this work, a mucus-binding factor knockout CRL1505 strain (ΔmbfCRL1505) was obtained and the lack of binding ability to both intestinal epithelial cells and mucin was demonstrated in vitro. Two sets of in vivo experiments in 6-week-old Balb/c mice were performed to evaluate ΔmbfCRL1505 immunomodulatory activities. A) Orally administered ΔmbfCRL1505 prior intraperitoneal injection of the TLR3 agonist poly(I:C) significantly reduced (p