Selection of porcine immunobiotics with the ability to improve innate antivaral immunity in porcine intestinal epithelial cells

INDO, YUHKI; MASUMIZU, YUKI; ZHOU, BINGHUI; AMINUL, MD ISLAM; MIYAZAKI, AYAKO; WAKAKO IKEDA-OHTSUBO; SUDA, YOSHIHITO; HISASHI ASO; JULIO VILLENA; HARUKI KITAZAWA

Congreso; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Rotavirus (RV) is a leading cause of viral gastroenteritis in young hosts, and immunobiotic lactobacilli are considered as effective alternatives to reduce RV diarrhea morbidity in humans. Since it is considered that host-derived immunobiotics would be more efficient to improve resistance to pathogens, immunobiotic lactobacilli of porcine origin are actively searched for the protection of young pigs against RV infection. This study evaluated the ability of porcine intestinal Lactobacillus salivarius isolates to differentially modulate TLR3-mediate innate immunity and protect against RV infection. For this purpose, porcine intestinal epithelial (PIE) cells were individually stimulated with eight L. salivarius strains for 48h and their adhesion ability was evaluated by using Biacore fluorescence assay. In addition, after lactobacilli stimulation for 48h, PIE cells were challenged with the TLR3-agonist poly(I:C) for 12h or RV strain OSU 12h and 16h, and the expression of type I and type III interferons (IFNs) and the resistance to viral infection was evaluated. Results showed a strain-dependent ability of lactobacilli to adhere to PIE cells, to improve IFNs expression, and reduce RV replication. Among the strain evaluated, L. salivarius FFIG58 showed the highest ability to adhere to porcine cells (p